GlycoSens™ β-Hydroxybutyrate Assay Kit, Enzymatic Method

The GlycoSens™ β-Hydroxybutyrate Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of β-hydroxybutyrate levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Other Series

β-Hydroxybutyrate

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

5 μL - 20 μL

Quantification of β-hydroxybutyrate is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

β-Hydroxybutyrate

β-Hydroxybutyrate

As a pivotal biomolecule, β-hydroxybutyrate plays a central role in complex physiological networks. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Notably, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Tailored for accurate quantification of β-hydroxybutyrate across cell biology, organelle bioenergetics, and intracellular signaling cascades.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.