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GlycoSens™ β-Hydroxybutyrate Assay Kit

CAT#: GLJF-0926-HX1251

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Specifications
Product Description
The GlycoSens™ β-Hydroxybutyrate Assay Kit offers an advanced quantitative methodology for evaluating β-hydroxybutyrate concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Basal Metabolic Series
Target
β-Hydroxybutyrate
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target β-hydroxybutyrate activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of β-hydroxybutyrate levels.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
β-Hydroxybutyrate
Alternate Names
β-Hydroxybutyrate
Application Notes
Background
β-Hydroxybutyrate acts as an essential biochemical entity within diverse cellular pathways. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Essential for monitoring β-hydroxybutyrate level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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