GlycoSens™ Lactate Dehydrogenase Isoenzyme 1 (LDH-1) Assay Kit

The GlycoSens™ Lactate Dehydrogenase Isoenzyme 1 (LDH-1) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of lactate dehydrogenase isoenzyme 1. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

LDH-1

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Target lactate dehydrogenase isoenzyme 1 (LDH-1) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target LDH-1.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Lactate Dehydrogenase Isoenzyme 1

Lactate Dehydrogenase Isoenzyme 1; LDH-1; Lactate Dehydrogenase Isoenzyme 1 Standard; Lactate Dehydrogenase Isoenzyme 1 Reagent

Lactate dehydrogenase isoenzyme 1 (LDH-1) acts as an essential biochemical entity within diverse cellular pathways. It catalyzes critical oxidation-reduction reactions by transferring hydride groups to specific electron acceptors. In addition, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Designed for comprehensive functional analysis of lactate dehydrogenase isoenzyme 1 (LDH-1) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Stable Signal Output; Streamlined Protocol; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.