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The GlycoSens™ Phosphoglucomutase (PGM) Assay Kit offers an advanced quantitative methodology for evaluating phosphoglucomutase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
The system measures chromatic product formation derived from target phosphoglucomutase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of PGM levels.
Detection Time
30-60 minutes
Detection Wavelength
450 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Phosphoglucomutase
Alternate Names
Phosphoglucomutase; PGM
Application Notes
Background
Research highlights phosphoglucomutase (PGM) as a significant participant in key metabolic and regulatory cascades. By lowering activation energy barriers, it drives essential chemical reactions that sustain cellular life. Moreover, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Designed for comprehensive functional analysis of phosphoglucomutase (PGM) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.