GlycoSens™ Phosphoglucomutase (PGM) Assay Kit

The GlycoSens™ Phosphoglucomutase (PGM) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target phosphoglucomutase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Carbohydrate Metabolism Series

PGM

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

5 μL - 20 μL

The assay relies on the distinct spectroscopic optical characteristics of phosphoglucomutase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect PGM content in the sample.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phosphoglucomutase

Phosphoglucomutase; PGM

The presence and activity of phosphoglucomutase (PGM) are closely linked to overall organismal health. Its enzymatic activity is finely tuned to environmental cues, reflecting the overall metabolic capacity. As a result, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Widely applicable for tracking phosphoglucomutase (PGM) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.