GlycoSens™ Phosphoglucomutase (PGM) Assay Kit, Microplate Method

The GlycoSens™ Phosphoglucomutase (PGM) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of phosphoglucomutase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Other Series

PGM

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Target phosphoglucomutase (PGM) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PGM.

30-60 minutes

450 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phosphoglucomutase

Phosphoglucomutase; PGM

As a pivotal biomolecule, phosphoglucomutase (PGM) plays a central role in complex physiological networks. It effectively accelerates specific biochemical transformations, ensuring the rapid progression of metabolic pathways. Furthermore, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Essential for monitoring phosphoglucomutase (PGM) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.