GlycoSens™ Pyruvate (PA) Assay Kit, Spectrophotometry

The GlycoSens™ Pyruvate (PA) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of pyruvate levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Tricarboxylic Acid Cycle Series

PA

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

20 μL - 100 μL

Quantification of pyruvate (PA) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pyruvate

Pyruvate; PA

Research highlights pyruvate (PA) as a significant participant in key metabolic and regulatory cascades. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. Consequently, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring pyruvate (PA) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.