GlycoSens™ Pyruvate (PA) Assay Kit, Micro-method

The GlycoSens™ Pyruvate (PA) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of pyruvate. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Tricarboxylic Acid Cycle Series

PA

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

Target pyruvate (PA) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PA.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pyruvate

Pyruvate; PA

Pyruvate (PA) acts as an essential biochemical entity within diverse cellular pathways. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains. Consequently, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.

Specifically engineered for evaluating pyruvate (PA) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.