GlycoSens™ Total Antioxidant Capacity (DPPH Method) Assay Kit

The GlycoSens™ Total Antioxidant Capacity Assay Kit utilizes a highly stable detection system tailored for accurate profiling of total antioxidant capacity. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

50 μL

This assay relies on the unique spectroscopic optical signature of total antioxidant capacity. After reacting with specialized chromogenic reagents, the measured optical density change directly reflects the total antioxidant capacity of the sample.

Within 1 hour

515 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

As a pivotal biomolecule, total antioxidant capacity plays a central role in complex physiological networks. It represents a comprehensive biomarker for assessing the aggregate biochemical capacity of the sample. As a result, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Essential for monitoring total antioxidant capacity level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Broad Sample Compatibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.