GlycoSens™ Total Protease Assay Kit, Micro-method

The GlycoSens™ Total Protease Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of total protease levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Other Series

Assay Buffer; Substrate Protein; Stop Solution; Standard Solution

Tissues, other biological samples

10 μL - 50 μL

Quantification of total protease is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

660 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Total protease is recognized as a fundamental component in biological systems. This enzyme plays a pivotal role in biological degradation, nutrient mobilization, and quality control. As a result, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Specifically engineered for evaluating total protease dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.