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GlycoSens™ Alkaline Phosphatase (AKP/ALP) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX484

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ Alkaline Phosphatase (AKP/ALP) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of alkaline phosphatase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Esterase Series
Target
AKP/ALP
Kit Components
Sample Lysis Buffer; Substrate; Detection Reagent; Standard
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
Target alkaline phosphatase (AKP/ALP) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target AKP/ALP.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Alkaline Phosphatase
Alternate Names
Alkaline Phosphatase; AKP/ALP; Alkaline Phosphohydrolase
Application Notes
Background
Functioning at the core of various biological processes, alkaline phosphatase (AKP/ALP) is a key subject of biochemical analysis. Its hydrolytic action on phosphate bonds is essential for energy metabolism and signal termination. Investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Tailored for accurate quantification of alkaline phosphatase (AKP/ALP) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
High Specificity; Stable Reaction; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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