GlycoSens™ Trehalose Assay Kit, Micro-method

The GlycoSens™ Trehalose Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target trehalose in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Trehalose Series

Trehalose

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of trehalose. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect trehalose content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalose

Trehalose

Trehalose is recognized as a fundamental component in biological systems. This carbohydrate molecule is central to carbon metabolism and cellular energy distribution. Moreover, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Ideal for investigating trehalose activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.