GlycoSens™ Trehalose Assay Kit, Anthrone Method

The GlycoSens™ Trehalose Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of trehalose levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Carbohydrate Metabolism Series

Trehalose

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

5 μL - 20 μL

Quantification of trehalose is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalose

Trehalose

The presence and activity of trehalose are closely linked to overall organismal health. It serves as a fundamental energy source and a key structural component in biological tissues. In addition, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Optimized for high-throughput profiling of trehalose in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.