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Quantitative Human IL4R (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX321

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Specifications
Product Description
The quantitative human IL4R (glycosylated) sandwich ELISA kit is designed to detect human interleukin 4 receptor (IL4R) levels. IL4R is a transmembrane protein, which binds to interleukin-4 (IL-4). This binding initiates intracellular signaling pathways. IL4R plays a role in regulating immune responses, which is involved in the differentiation of T cells. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 6.836 pg/mL, which can accurately detect low concentrations of IL4R in the sample.
Target
IL4R
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
6.836 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interleukin 4 receptor
Alternate Names
IL4R; Interleukin 4 receptor; CD124; IL4RA; IL-4-binding protein
Uniprot No.
N-Glycosylation Site
53, 98, 128, 134, 176, 209
Application Notes
Application
The quantitative human IL4R (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated IL4R levels. It is useful for studying the role of IL4R in immune regulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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