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Quantitative Human vWFCP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX659)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human vWFCP (glycosylated) sandwich ELISA kit is designed to detect human von willebrand factor cleaving protease (vWFCP) levels. vWFCP is a metalloprotease enzyme. This enzyme specifically cleaves von Willebrand factor (vWF), a large glycoprotein involved in blood clotting. vWFCP regulates the size and activity of vWF, preventing the formation of excessively large blood clots. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.145 ng/mL, which can accurately detect low concentrations of vWFCP in the sample.
Target vWFCP
N-Glycosylation Site 142, 146, 552, 579, 614, 667, 707, 828, 1235, 1354
O-Glycosylation Site 399, 698, 757, 907, 965, 1027, 1087
C-Glycosylation Site 387
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.145 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Von willebrand factor cleaving protease
Alternate Names vWFCP; Von willebrand factor cleaving protease; ADAMTS13; VWF-cleaving protease; A disintegrin and metalloproteinase with A thrombospondin type 1 motif member 13
Uniprot No. Q76LX8
Application The quantitative human vWFCP (glycosylated) sandwich ELISA kit is used to determine the vWFCP levels in biological samples. The kit is applicable in studies of blood coagulation, von Willebrand factor regulation, and thrombotic disorders. It is useful in fields such as hematology and vascular biology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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