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Quantitative Mouse IFN-γ (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX494

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative mouse IFN-γ (glycosylated) sandwich ELISA kit is designed to detect mouse interferon γ (IFN-γ) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 4.215 pg/mL, which can accurately detect low concentrations of IFN-γ in the sample.
Target
IFN-γ
N-Glycosylation Site
38, 90
Sample Types
Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
4.215 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
16 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Interferon γ
Alternate Names
IFN-γ; Interferon gamma; IFN-gamma
Uniprot No.
P01580
Application Notes
Application
The quantitative mouse IFN-γ (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of IFN-γ, helping to understand its role in immunology.
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated IFN-γ antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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