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Quantitative Mouse TRACP-5b (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0125-HX26

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative mouse TRACP-5b (glycosylated) sandwich ELISA kit is designed to detect mouse tartrate-resistant acid phosphatase 5b(TRACP-5b) protein levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.022 mIU/mL, which can accurately detect low concentrations of TRACP-5b in the sample.
Target
ACP5
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated TRACP-5b antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.022 mIU/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.08 mIU/mL-5 mIU/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Acid phosphatase 5, tartrate resistant
Alternate Names
Tartrate-resistant acid phosphatase 5b; Acp5; Tartrate-resistant acid phosphatase type 5; TR-AP; Type 5 acid phosphatase
Uniprot No.
Q05117
N-Glycosylation Site
118, 149
Application Notes
Application
The quantitative mouse TRACP-5b (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of ACP5, helping to understand its role in signal transduction and related physiological processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-102%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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