GlycoSens™ Acid Phosphatase (ACP) Assay Kit, Microplate Method

The GlycoSens™ Acid Phosphatase (ACP) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of acid phosphatase (ACP) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

ACP

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Cell lysates, biological fluids

10 μL - 50 μL

Quantification of acid phosphatase (ACP) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acid Phosphatase

Acid Phosphatase; ACP; Acid Phosphohydrolase

Functioning at the core of various biological processes, acid phosphatase (ACP) is a key subject of biochemical analysis. Its hydrolytic action on phosphate bonds is essential for energy metabolism and signal termination. As a result, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Ideal for investigating acid phosphatase (ACP) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.