GlycoSens™ Acid Protease (ACP) Assay Kit, Spectrophotometry

The GlycoSens™ Acid Protease (ACP) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target acid protease in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Protease Series

ACP

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of acid protease. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect ACP content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acid Protease

Acid Protease; ACP

Functioning at the core of various biological processes, acid protease (ACP) is a key subject of biochemical analysis. Its hydrolytic activity is essential for maintaining the balance of the cellular and extracellular matrix. Moreover, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Specifically engineered for evaluating acid protease (ACP) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Linear Dynamic Range; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.