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The GlycoSens™ Acid Phosphatase (ACP) Assay Kit offers an advanced quantitative methodology for evaluating acid phosphatase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
The system measures chromatic product formation derived from target acid phosphatase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of ACP levels.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Acid Phosphatase
Alternate Names
Acid Phosphatase; ACP; Acid Phosphohydrolase
Application Notes
Background
Acid phosphatase (ACP) acts as an essential biochemical entity within diverse cellular pathways. This enzyme enables the dephosphorylation of target molecules, fundamentally altering their functional state. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Designed for comprehensive functional analysis of acid phosphatase (ACP) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
Key Features & Advantages
Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.