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GlycoSens™ Adenosine Triphosphate (ATP) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX8

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Specifications
Product Description
The GlycoSens™ Adenosine Triphosphate (ATP) Assay Kit enables precise, direct detection of ATP via spectrophotometric measurement. The kit contains ready-to-use reagents optimized for exceptional reproducibility and fast assay turnaround.
Product Type
Oxidative Phosphorylation Series
Target
ATP
Kit Components
Reference Standard; Extraction Solvent; Deproteinization Buffer
Sample Types
Tissue homogenates, cell lysates, biological fluids
Sample Volume
10 μL - 50 μL
Detection Principle
ATP exhibits a characteristic absorption peak at 254 nm. Sample ATP concentration is accurately measured using HPLC chromatographic separation and UV detection.
Detection Time
Within 1 hour
Detection Wavelength
254 nm
Required Equipment & Materials (Self-provided by User)
High-performance liquid chromatograph, centrifuge, chromatographic column, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Adenosine Triphosphate
Alternate Names
Adenosine Triphosphate; ATP; Adenosine Triphosphate Standard; Adenosine Triphosphate Reagent
Application Notes
Background
In the context of biological research, adenosine triphosphate (ATP) has emerged as a molecule of high interest. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains. Consequently, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Specifically engineered for evaluating adenosine triphosphate (ATP) dynamics in cell biology, organelle bioenergetics, and cellular energy coupling research.
Key Features & Advantages
High-Resolution Quantification; Rapid Reaction Protocol; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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