GlycoSens™ Adenosine Triphosphate (ATP) Assay Kit, Micro-method

The GlycoSens™ Adenosine Triphosphate (ATP) Assay Kit enables precise, direct detection of ATP via micro-volume spectrophotometric measurement. The kit contains ready-to-use reagents optimized for exceptional reproducibility and fast assay turnaround.

Oxidative Phosphorylation Series

ATP

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Creatine kinase catalyzes the reaction between ATP and creatine to yield phosphocreatine. Phosphocreatine content is determined colorimetrically via phosphomolybdate formation at 700 nm.

Within 1 hour

700 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Adenosine Triphosphate

ATP; Adenosine Triphosphate Standard; Adenosine Triphosphate Reagent; Adenosine 5'-Triphosphate

Adenosine triphosphate (ATP) is recognized as a fundamental component in biological systems. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Notably, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Essential for monitoring adenosine triphosphate (ATP) level shifts during physiological stress responses, cellular energy pool dynamics, and metabolic homeostasis research.

Stable Signal Output; Excellent Reproducibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.