GlycoSens™ DPPH Radical Scavenging Capacity Assay Kit

The GlycoSens™ DPPH Radical Scavenging Capacity Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of DPPH radical scavenging capacity levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

Reagent Solution; Assay Diluent; Standard Antioxidant ; Reaction Buffer

Extracts, synthetic antioxidant compounds

10 μL - 50 μL

Quantification of dpph radical scavenging capacity is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

517 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Found widely across biological samples, DPPH radical scavenging capacity is crucial for maintaining systemic homeostasis. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Tailored for accurate quantification of DPPH radical scavenging capacity across cell biology, organelle bioenergetics, and intracellular signaling cascades.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.