GlycoSens™ Nitrite Assay Kit, Micro-method

The GlycoSens™ Nitrite Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of nitrite levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Nitrogen Metabolism Series

Nitrite

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

Quantification of nitrite is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Nitrite

Nitrite

As a pivotal biomolecule, nitrite plays a central role in complex physiological networks. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Notably, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Tailored for accurate quantification of nitrite across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.