GlycoSens™ Polyphenol Oxidase (PPO) Assay Kit, Spectrophotometry

The GlycoSens™ Polyphenol Oxidase (PPO) Assay Kit offers an advanced quantitative methodology for evaluating polyphenol oxidase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Oxidation and Antioxidant Series

PPO

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissue homogenates, cellular lysates, microbial extracts

10 μL - 50 μL

The system measures chromatic product formation derived from target polyphenol oxidase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of PPO levels.

30-60 minutes

525 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Polyphenol Oxidase

Polyphenol Oxidase; PPO; Polyphenol Oxidoreductase

Polyphenol oxidase (PPO) is an integral element required for robust cellular function and metabolism. Through its catalytic activity, it ensures the safe processing of oxidative metabolites within the cell. Moreover, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Specifically engineered for evaluating polyphenol oxidase (PPO) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.