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The GlycoSens™ Soil Acid Protease (S-ACPT) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of soil acid protease. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Target soil acid protease (S-ACPT) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target S-ACPT.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Soil Acid Protease
Alternate Names
Soil Acid Protease; S-ACPT
Application Notes
Background
The presence and activity of soil acid protease (S-ACPT) are closely linked to overall organismal health. Its dynamics closely reflect soil fertility, organic matter decomposition, and ecosystem health. Furthermore, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.
Application
Specifically engineered for evaluating soil acid protease (S-ACPT) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.