GlycoSens™ Soil Dehydrogenase (SDHA) Assay Kit, Micro-method

The GlycoSens™ Soil Dehydrogenase (SDHA) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of soil dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Soil Series

SDHA

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Soil, sediments, solid substrates

10 μL - 50 μL

Target soil dehydrogenase (SDHA) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target SDHA.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Soil Dehydrogenase

Soil Dehydrogenase; SDHA; Soil Oxidoreductase

Soil dehydrogenase (SDHA) is an integral element required for robust cellular function and metabolism. It acts as a primary indicator of soil microbial activity and biogeochemical cycling. Assessing this component offers a reliable parameter for agricultural research or biological studies.

Essential for monitoring soil dehydrogenase (SDHA) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.