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Quantitative Human ESAM (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX728

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Specifications
Product Description
The quantitative human ESAM (glycosylated) sandwich ELISA kit is designed to detect human endothelial cell adhesion molecule (ESAM) levels. ESAM is a protein, which is located on endothelial cells. ESAM plays a role in cell adhesion. It is involved in the formation of blood vessels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of ESAM in the sample.
Target
ESAM
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.128 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Endothelial cell adhesion molecule
Alternate Names
ESAM; Endothelial cell adhesion molecule
Uniprot No.
N-Glycosylation Site
108, 169, 213, 236
Application Notes
Application
The quantitative human ESAM (glycosylated) sandwich ELISA kit is useful for measuring ESAM levels in samples. This measurement is relevant to vascular biology and angiogenesis. The kit also has potential applications in investigations of cardiovascular diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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