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Quantitative Human LOX (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX346

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Specifications
Product Description
The quantitative human LOX (glycosylated) sandwich ELISA kit is designed to detect human lysyl oxidase (LOX) levels. LOX is an enzyme, which catalyzes the crosslinking of collagen and elastin. LOX plays a crucial role in the formation of the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.103 ng/mL, which can accurately detect low concentrations of LOX in the sample.
Target
LOX
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.103 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lysyl oxidase
Alternate Names
LOX; Lysyl oxidase; Protein-lysine 6-oxidase
Uniprot No.
N-Glycosylation Site
81, 97, 144
Application Notes
Application
The quantitative human LOX (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LOX levels. It is useful for studying the role of LOX in collagen crosslinking.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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