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Quantitative Human PON2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX599

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Specifications
Product Description
The quantitative human PON2 (glycosylated) sandwich ELISA kit is designed to detect human paraoxonase 2 (PON2) levels. PON2 is an enzyme with antioxidant properties, which is known to protect against oxidative stress by hydrolyzing organophosphates. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.352 ng/mL, which can accurately detect low concentrations of PON2 in the sample.
Target
PON2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.352 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.25 ng/mL-40 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Paraoxonase 2
Alternate Names
PON2; Paraoxonase 2; Aromatic esterase 2
Uniprot No.
N-Glycosylation Site
254, 269, 323
Application Notes
Application
The quantitative human PON2 (glycosylated) sandwich ELISA kit provides a quantitative method for measuring glycosylated PON2 levels in various biological samples. It is applicable in studies related to oxidative stress, cardiovascular disease, and toxicology. The kit is valuable in fields such as cardiology, environmental health, and studies on the effects of organophosphate exposure.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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