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Quantitative Human SIGLEC2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX283

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Specifications
Product Description
The quantitative human SIGLEC2 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding Ig like lectin 2 (SIGLEC2) levels. SIGLEC2 is a protein that is found on the surface of B cells. This protein binds to sialic acids, which plays a role in B cell signaling and immune regulation. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 1.529 ng/mL, which can accurately detect low concentrations of SIGLEC2 in the sample.
Target
SIGLEC2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
1.529 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
5 ng/mL-150 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sialic acid binding Ig like lectin 2
Alternate Names
SIGLEC2; Sialic acid binding Ig like lectin 2; CD22; BL-CAM; B-cell receptor CD22; B-Lymphocyte cell adhesion molecule
Uniprot No.
N-Glycosylation Site
67, 101, 112, 135, 164, 231, 363, 445, 479, 574, 634
Application Notes
Application
The quantitative human SIGLEC2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated SIGLEC2 levels in biological samples. It can be used in studies examining autoimmune diseases, B cell lymphomas, and inflammation. The kit is applicable in immunological research.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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