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Quantitative Human TLR7 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX857

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Specifications
Product Description
The quantitative human TLR7 (glycosylated) sandwich ELISA kit is designed to detect human toll like receptor 7 (TLR7) levels. TLR7 is a pattern recognition receptor that is located in endosomes and recognizes single-stranded RNA. TLR7 plays a role in the innate immune system. This receptor is involved in activating immune responses to viral infections. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.427 ng/mL, which can accurately detect low concentrations of TLR7 in the sample.
Target
TLR7
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.427 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.50 ng/mL-45 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Toll like receptor 7
Alternate Names
TLR7; Toll like receptor 7; CD287
Uniprot No.
N-Glycosylation Site
66, 69, 167, 202, 215, 361, 413, 488, 523, 534, 590, 679, 720, 799
Application Notes
Application
The quantitative human TLR7 (glycosylated) sandwich ELISA kit is used to measure TLR7 levels in biological samples. This kit is applicable in studies examining innate immunity, viral infections, and autoimmune diseases. It is also relevant to studies investigating the role of TLR7 in the recognition of RNA viruses and the subsequent activation of immune responses.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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