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Quantitative Rat NE (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX801)

Datasheet
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1×96 T
5×96 T
10×96 T

Product Description The quantitative rat NE (glycosylated) sandwich ELISA kit is designed to detect rat neutrophil elastase (NE) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 3.425 ng/mL, which can accurately detect low concentrations of NE in the sample.
Target NE
N-Glycosylation Site 88, 124, 173
Sample Types Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume 50 μL-100 μL
Sensitivity 3.425 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 15 ng/mL-800 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Rat
Full Name Neutrophil elastase
Alternate Names Neutrophil elastase; Bone marrow serine protease; ELA2; Elastase 2; GE; Granulocyte derived elastase; HLE; Human leukocyte elastase; Leukocyte elastase; PMN elastase; Polymorphonuclear elastase; NE
Uniprot No. P08246
Application The quantitative rat NE (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of NE, helping to understand its role in signal transduction.
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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