GlycoErase™ ALG12 Knockout CHO-S Cell Line (CAT#: GLJF-0425-JF130)

Description	To deeply analyze the role of terminal glucose in N-glycosylation, particularly regarding the impact of Glc3 trimming on glycoprotein quality and endoplasmic reticulum quality control, the ALG10 knockout CHO-S cell line is designed as an ideal model to serve the optimization of therapeutic protein drug production in CHO-S cells.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, suspension growth
Passage Ratio	1:3~1:5
Cell Line	CHO-S
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	CDMF & Glutamine & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ALG12
Full Name	ALG12 alpha-1,6-mannosyltransferase
Alternative Name	ECM39, CDG1G
Location	22q13.33
Gene ID	79087
Summary	This gene encodes a protein that belongs to the glycosyltransferase 22 family. The protein plays a crucial role in protein glycosylation by catalyzing the addition of the eighth mannose residue to the dolichol pyrophosphate-linked oligosaccharide precursor (dolichol-PP-Man₇GlcNAc₂). This addition occurs via an alpha-1,6 linkage, and is essential for the proper assembly of the oligosaccharide chain required for N-linked glycoprotein synthesis.