Engineered to facilitate research on N-linked glycosylation, this ALG12 knockout HEK293T cell line specifically targets the alpha-1,6-mannosyltransferase responsible for adding the eighth mannose residue to dolichol-PP-oligosaccharides. Researchers can utilize this model to explore the effects of disrupting this crucial step in N-glycan biosynthesis, gaining insights into the impact of specific N-glycan structures on protein function and cellular processes.

Product Type

KO Cell Lines

Species

Human

Cell Morphology

Epithelial-like, adherent

Passage Ratio

1:2~1:4

Cell Line

HEK293T

Lineage

Embryonic kidney

Specification

Cell Viability

>90%

Sterility Test

The sterility test indicated an absence of microbial growth.

Identity Test

STR identification

Mycoplasma Test

Negative

Virus Test

Negative for HIV, HBV and HCV.

Genetic Stability Testing

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

Validation

PCR, Sanger Sequencing

Culture Medium

DMEM & FBS & Penicillin/Streptomycin

Application

Functional assay

Size

1 M cells/vial*2

Product Format

Frozen

Shipping

Dry ice

Availability Status

Made to order

Handling Notes

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

Product Disclaimer

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target Information

Target

ALG12

Full Name

ALG12 alpha-1,6-mannosyltransferase

Alternative Name

ECM39, CDG1G

Location

22q13.33

Gene ID

79087

Summary

This gene encodes a protein that belongs to the glycosyltransferase 22 family. The protein plays a crucial role in protein glycosylation by catalyzing the addition of the eighth mannose residue to the dolichol pyrophosphate-linked oligosaccharide precursor (dolichol-PP-Man₇GlcNAc₂). This addition occurs via an alpha-1,6 linkage, and is essential for the proper assembly of the oligosaccharide chain required for N-linked glycoprotein synthesis.

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For Research Use Only.

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