GlycoErase™ SIGLEC2 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF882)

Description	GlycoErase™ SIGLEC2 knockout HEK293 cell line is a human embryonic kidney cell line lacking SIGLEC2 expression. SIGLEC2 acts as a sialic acid-binding receptor involved in CD4 receptor interactions, B-cell activation, receptor signaling, and endocytosis, and it has been linked to diffuse large B-cell lymphoma. This knockout HEK293 model offers a useful platform for investigating immune receptor pathways, endosomal trafficking, and B-cell-related mechanisms, with applications in functional genomics, antibody research, and viral studies.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC2
Full Name	Sialic Acid Binding Ig Like Lectin 2
Alternative Name	SIGLEC-2, CD22, Sialic Acid Binding Ig Like Lectin
Location	19q13.12
Gene ID	8778
Summary	This protein is predicted to bind CD4 receptors, protein phosphatases, and sialic acid. It is involved in the activation of B cells, suppression of B cell receptor signaling, and the regulation of endocytosis. The protein is found in early and recycling endosomes and is associated with diffuse large B-cell lymphoma.