GlycoErase™ SIGLEC2 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF881)

Description	GlycoErase™ SIGLEC2 knockout CHO-K1 cell line is a Chinese hamster ovary cell line lacking SIGLEC2 expression. SIGLEC2 functions as a sialic acid-binding receptor involved in CD4 receptor interactions, B-cell activation, receptor signaling, and endocytosis, and it is implicated in diffuse large B-cell lymphoma. The loss of SIGLEC2 in the CHO-K1 background creates a distinctive model to investigate immune receptor pathways, vesicular trafficking, and B-cell-related mechanisms, with applications in antibody discovery, preclinical evaluation, and glycoengineering.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC2
Full Name	Sialic Acid Binding Ig Like Lectin 2
Alternative Name	SIGLEC-2, CD22, Sialic Acid Binding Ig Like Lectin
Location	19q13.12
Gene ID	8778
Summary	The protein is predicted to exhibit CD4 receptor binding activity, protein phosphatase binding activity, and sialic acid binding activity. It is involved in B cell activation, negative regulation of the B cell receptor signaling pathway, and regulation of endocytosis. The protein is located in early and recycling endosomes and is implicated in diffuse large B-cell lymphoma.