GlycoErase™ SIGLEC2 Knockout CHO-K1 Cell Line

GlycoErase™ SIGLEC2 knockout CHO-K1 cell line is a Chinese hamster ovary cell line lacking SIGLEC2 expression. SIGLEC2 functions as a sialic acid-binding receptor involved in CD4 receptor interactions, B-cell activation, receptor signaling, and endocytosis, and it is implicated in diffuse large B-cell lymphoma. The loss of SIGLEC2 in the CHO-K1 background creates a distinctive model to investigate immune receptor pathways, vesicular trafficking, and B-cell-related mechanisms, with applications in antibody discovery, preclinical evaluation, and glycoengineering.

KO Cell Lines

Hamster

Epithelial, adherent

1:3~1:5

CHO-K1

Chinese hamster ovary

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC2

Sialic Acid Binding Ig Like Lectin 2

SIGLEC-2, CD22, Sialic Acid Binding Ig Like Lectin

The protein is predicted to exhibit CD4 receptor binding activity, protein phosphatase binding activity, and sialic acid binding activity. It is involved in B cell activation, negative regulation of the B cell receptor signaling pathway, and regulation of endocytosis. The protein is located in early and recycling endosomes and is implicated in diffuse large B-cell lymphoma.