Description

GlycoFlux™ MMLV-ALG3 Viral Particle provides ALG3, the α1,3-mannosyltransferase that extends Man₅GlcNAc₂-PP-Dol; loss of function is linked to CDG-Id with glycosylation defects and neurological features. It enables construction of stably transduced mammalian cells to model early LLO elongation, perform genotype-phenotype rescue, and screen ER-glycosylation modulators.

Product Type

Recombinant MMLV Retrovirus

Target

ALG3

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Full Name	Alg3 Alpha-1,3- Mannosyltransferase
Alternative Name	NOT56L; D16Ertd36e; CDGS4; Not56
Location	3q27.1
Gene ID	10195
Summary	Critical in N-glycan assembly, transferring alpha-1,3 mannose to Man5GlcNAc2-PP-Dol; loss of function is linked to CDG-Id, which disrupts glycosylation and may cause neurological disorders.

GlycoFlux™ MMLV-Human ALG3 Viral Particle (CAT#: GLJF-1025-JF261)

Specifications

Quality Control

Target Information

Related products