GlycoSens™ Cysteine (Cys) Assay Kit, Colorimetry

The GlycoSens™ Cysteine (Cys) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target cysteine in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

Cys

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Cell lysates, tissue homogenates

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of cysteine. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect Cys content in the sample.

Within 1 hour

600 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Cysteine

Cysteine; Cys

Cysteine (cys) acts as an essential biochemical entity within diverse cellular pathways. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Notably, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Optimized for high-throughput profiling of cysteine (Cys) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.