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The GlycoSens™ Cysteine (Cys) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of cysteine (Cys) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Nitrogen Metabolism Series
Target
Cys
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
Quantification of cysteine (Cys) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Cysteine
Alternate Names
Cysteine; Cys
Application Notes
Background
Functioning at the core of various biological processes, cysteine (Cys) is a key subject of biochemical analysis. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. In addition, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Designed for comprehensive functional analysis of cysteine (Cys) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.