GlycoSens™ Phytase Assay Kit, Spectrophotometry

The GlycoSens™ Phytase Assay Kit utilizes a highly stable detection system tailored for accurate profiling of phytase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Other Series

Phytase

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

Target phytase in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target phytase.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phytase

Phytase

Phytase acts as an essential biochemical entity within diverse cellular pathways. It selectively processes distinct biochemical substrates, maintaining the efficiency of cellular networks. Consequently, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring phytase level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.