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Quantitative Human FPN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX829)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human FPN (glycosylated) sandwich ELISA kit is designed to detect human ferroportin (FPN) levels. FPN is a transmembrane protein that is expressed in various cell types, including enterocytes and macrophages. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of FPN in the sample.
Target FPN
N-Glycosylation Site 434
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ferroportin
Alternate Names FPN; Ferroportin; SLC40A1; FPN1; Solute carrier family 40, member 1
Uniprot No. Q9NP59
Application The quantitative human FPN (glycosylated) sandwich ELISA kit is used to measure FPN levels in biological samples. This kit is applicable in studies examining iron transport, iron deficiency, and iron overload disorders. It is also relevant to studies investigating the role of FPN in regulating systemic iron levels.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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