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Quantitative Human IL12A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX357)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IL12A (glycosylated) sandwich ELISA kit is designed to detect human interleukin 12A (IL12A) levels. IL12A is a subunit of interleukin-12 (IL-12). IL-12 is a cytokine, which plays a role in stimulating T cell and NK cell activity. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 3.592 pg/mL, which can accurately detect low concentrations of IL12A in the sample.
Target IL12A
N-Glycosylation Site 93, 107
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 3.592 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 10 pg/mL-500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Interleukin 12A
Alternate Names IL12A; Interleukin 12A; IL-12A; CLMF1; NKSF1; Natural killer cell stimulatory factor 1; Cytotoxic lymphocyte maturation factor 1
Uniprot No. P29459
Application The quantitative human IL12A (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated IL12A levels. It is useful for studying the role of IL-12 in promoting cellular immunity.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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