Recombinant Measles virus (MV) (strain Halle) Hemagglutinin glycoprotein (H) (1-617 aa), E. coli

Recombinant Measles virus (MV) (strain Halle) Hemagglutinin glycoprotein (1-617 aa) was expressed in E. coli with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Measles virus (MV) (strain Halle)

1-617 aa

MSPQRDRINAFYKDNPHPKGSRIVINREHLMIDRPYVLLAVLFVMFLSLIGLLAIAGIRL HRAAIYTAEIHKSLSTNLDVTNSIEHQVKDVLTPLFKIIGDEVGLRTPQRFTDLVKFISD KIKFLNPDREYDFRDLTWCINPPERIKLDYDQYCADVAAEELMNALVNSTLLETRTTNQF LAVSKGNCSGPTTIRGQFSNMSLSLLDLYLGRGYNVSSIVTMTSQGMYGGTYPVEKPNLS SKRSELSQLSMYRVFEVSVIRNPGLGAPVFHMTNYLEQPVSNDLSNCMVALGELKLAALC HGEDSITIPYQGSGKGVSFQLVKLGVWKSPTGMQSWVPLSTDDPVIDRLYLSSHRGVIAD NQAKWAVPTTRTDDKLRMETCFQQACKGKIQALCENPECVPLKDNRIPSYGVLSVDLSLT VELKIKIASGFGPLITHGSGMDLYKSNHNNVYWLTIPPMKNLALGVINTLEWIPRFKVSP YLFTVPIKEAGEDCHAPTYLPAEVDGDVKLSSNLVILPGQDLQYVLATYDTSRVEHAVVY YVYSPGRSFSYFYPFRLPIKGVPIELQVECFTWDQKLWCRHFCVLADSESGGHITHSGMV GMGVSCTVTREDGTNRR

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

H

Hemagglutinin glycoprotein

Attaches the virus to cell receptors and thereby initiating infection. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. May use human CD46 and/or SLAMF1 as receptors for viral entry into the cell. The high degree of interaction between H and MCP/CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis.

Hemagglutinin glycoprotein