Recombinant Viral hemorrhagic septicemia virus (VHSV) (strain 07-71) Spike glycoprotein (G) (21-507 aa), E. coli

Recombinant Viral hemorrhagic septicemia virus (VHSV) (strain 07-71) Spike glycoprotein (21-507 aa) was expressed in E. coli with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Viral hemorrhagic septicemia virus (VHSV) (strain 07-71)

21-507 aa

QITQRPPVENISTYHADWDTPLYTHPSNCRDDSFVPIRPAQLRCPHEFEDINKGLVSVPT RIIHLPLSVTSVSAVASGHYLHRVTYRVTCSTSFFGGQTIEKTILEAKLSRQEATDEASK DHEYPFFPEPSCIWMKNNVHKDITHYYKTPKTVSVDLYSRKFLNPDFIEGVCTTSPCQTH WQGVYWVGATPKAHCPTSETLEGHLFTRTHDHRVVKAIVAGHHPWGLTMACTVTFCGTEW IKTDLGDLIQVTGPGGTRKLTPNKCVNTDIQMRGATDDFSYLNHLITNMAQRTECLDAHS DITASGKVSSFLLSKFRPSHPGPGKAHYLLDGQIMRGDCDYEAVVSINYNRAQYKTMNNT WKSWKRVDNNTDGYDGMIFGDKLIIPDIEKYQSVYDSGMLVQRNLVEVPHLSIVFVSNTS DLSTNHIHTNLIPSDWSFNWSLWPSLSGMGVVGGAFLLLVLCCCCKASPPIPNYGIPMQQ FSRSQTV

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

G

Spike glycoprotein

This protein forms spikes on the surface of the virion. It is responsible both for the binding of the virus to susceptible host cells and for inducing the uptake of the virus by the cell. The interaction between the internal components of the virion and the portion of the glycoprotein exposed on the cytoplasmic face of the plasma membrane probably directs envelopment and virus budding.

Spike glycoprotein