GlycoSens™ Total Protease Assay Kit, Spectrophotometry

The GlycoSens™ Total Protease Assay Kit offers an advanced quantitative methodology for evaluating total protease concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Other Series

Extraction Buffer; Assay Buffer; Substrate Solution; Standard

Tissues, other biological samples

10 μL - 50 μL

The system measures chromatic product formation derived from target total protease activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of total protease levels.

Within 1 hour

660 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Total protease is an integral element required for robust cellular function and metabolism. This enzyme plays a pivotal role in biological degradation, nutrient mobilization, and quality control. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Tailored for accurate quantification of total protease across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.