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This biotinylated phosphatidylethanolamine (PE) is a functionalized derivative where the biotin group is covalently linked to the phospholipid structure. PE is a major structural component, synthesized predominantly via the CDP-ethanolamine pathway, and comprises a significant proportion of total membrane lipids. It is preferentially localized in the inner leaflet of eukaryotic cell membranes and is abundant in bacterial membranes.