GlycoErase™ SIGLEC2 Knockout A549 Cell Line (CAT#: GLJF-0825-JF880)

Description	GlycoErase™ SIGLEC2 knockout A549 cell line is a human lung epithelial cancer cell line in which SIGLEC2 expression has been eliminated. SIGLEC2 functions as a sialic acid-binding adhesion molecule involved in CD4 receptor interactions, B-cell activation, regulation of receptor signaling, and endocytosis, and it is also implicated in diffuse large B-cell lymphoma. The loss of SIGLEC2 in the A549 background creates a distinctive model for investigating immune receptor pathways, endosomal trafficking, and B-cell-related disease mechanisms, and provides a useful platform for preclinical drug evaluation and antibody research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC2
Full Name	Sialic Acid Binding Ig Like Lectin 2
Alternative Name	SIGLEC-2, CD22, Sialic Acid Binding Ig Like Lectin
Location	19q13.12
Gene ID	8778
Summary	Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome. Implicated in diffuse large B-cell lymphoma.