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Glycoprotein Deglycosylation Kit (Hydrazinolysis Reaction)

CAT#: GLJF-0125-HX108

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The glycoprotein deglycosylation kit (hydrazinolysis reaction) uses the chemical reaction between hydrazine and N-linked and O-linked sugar chains in glycoproteins to release these glycans from glycoproteins. This process usually involves the cleavage of glycosidic bonds, allowing the glycans to be released in a free form. These released glycans have free reducing ends and can be fluorescently labeled by reductive amination to enable subsequent detection and analysis. One kit contains deglycosylation of 12 glycoprotein samples.
Sample Types
Glycoproteins
Detection Principle
Chemical reaction
Storage
Store at 2-8°C for long term storage.
Target Information
Full Name
Glycoproteins
Application Notes
Application
The glycoprotein deglycosylation kit (hydrazinolysis reaction) is designed primarily for the separation of N-linked and O-linked glycans from glycoprotein biologics.
Kit Components
Hydrazinolysis release solution; Acetic anhydride; TFA solution; Octanol; Sodium bicarbonate; Wash solution; Cartridges
For Research Use Only.
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