GlycoSens™ Total Protease Assay Kit

The GlycoSens™ Total Protease Assay Kit utilizes a highly stable detection system tailored for accurate profiling of total protease. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Basal Metabolic Series

Assay Buffer; Substrate; Stop Solution; Standard

Tissues, other biological samples

10 μL - 50 μL

Target total protease in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target total protease.

Within 1 hour

660 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

As a pivotal biomolecule, total protease plays a central role in complex physiological networks. By selectively hydrolyzing complex molecules, it regulates the availability of active biological monomers. Furthermore, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Tailored for accurate quantification of total protease across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.